ABSTRACT
The emergence and spread of carbapenem resistance in Gram-negative bacilli such as Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa through the production of carbapenemases is a global phenomenon. It threatens patient care and leads to therapeutic impasses. This study aims to genotypically determine the prevalence of the most frequent carbapenemase genes among multidrug-resistant E. coli strains isolated from patients at a biomedical analysis laboratory. A total of fifty-three unduplicated E. coli strains isolated from patient samples with a multidrug-resistant (MDR) profile were subjected to polymerase chain reaction (PCR) testing for carbapenem resistance genes. This study allowed us to identify fifteen strains carrying resistance genes among the fifty-three E. coli strains. All fifteen strains produced the metallo-ß-lactamase enzymes; this represents a rate of 28.30% of study strains. Among these strains, ten carried the NDM resistance gene, NDM and VIM genes were detected in three strains and VIM was identified in two strains of E. coli. However, carbapenemases A (KPC and IMI), D (OXA-48), and IMP were not detected in the strains studied. Thus, NDM and VIM are the main carbapenemases detected in the strains in our study.
ABSTRACT
Introduction: la qualité des farines infantiles utilisées pendant la période de diversification alimentaire est d´une grande importance. Malheureusement, les processus de transformation occasionnent des pratiques d'hygiène insuffisantes. L´objectif de cette étude était d´évaluer la qualité physico-chimique et microbiologique des farines infantiles locales vendues à Ouagadougou. Méthodes: les paramètres physico-chimiques et microbiologiques ont été déterminés selon les méthodes standards. Les données ont été traitées sur Excel 2016 et les moyennes comparées sur XLSTAT 2016. Résultats: sur 102 unités de 20 marques de farines infantiles locales analysées, les matières premières de base étaient constituées par les céréales locales, les légumineuses, les enzymes, les minéraux et les vitamines. La teneur en eau variait de 1,92±0,01% à 5,51±0,03% tandis que le pH variait de 5,55±0,01 à 6,36±0,00. La flore totale variait de 2,4.102 UFC/g à 1,1.104 UFC/g, les coliformes totaux de 0 à 2,8.103 UFC/g, les coliformes fécaux de 0 à 5,3.102UFC/g et les levures et moisissures de 4 UFC/g à 1,1.103UFC/g. Aucune colonie confirmée de salmonelles et d´Escherichia coli n´a été détectée. Concernant l´évaluation microbiologique, toutes les farines à cuire ont présenté une flore aérobie totale, des Escherichia coli et des salmonella satisfaisantes, 64,71% ont présenté des coliformes fécaux satisfaisantes et 94,12% ont présenté des levures et moisissures satisfaisantes. Toutes les farines instantanées ont présenté des charges microbiologiques satisfaisantes. Conclusion: globalement, les farines infantiles locales vendues à Ouagadougou sont de qualité physico-chimiques et microbiologiques satisfaisantes à l´exception de quelques farines à cuire.
Introduction: the quality of infant flours used to support dietary diversification is of great importance. Unfortunately, transformation processes result in poor hygiene practices. The purpose of this study was to evaluate the physicochemical and microbiological quality of local infant flours sold in Ouagadougou. Methods: physicochemical and microbiological parameters were determined according to standard methods. Data were processed on Excel 2016 and the averages were compared using XLSTAT 2016. Results: out of the 102 units of 20 local infant flour brands analyzed, the basic raw materials consisted of local cereals, legumes, enzymes, minerals and vitamins. Water content ranged from 1.92±0.01% to 5.51±0.03%, while pH ranged from 5.55±0.01 to 6.36±0.00. Total flora ranged from 2,4.102 CFU/g to 1,1.104 CFU/g, total coliforms from 0 to 2,8.103 CFU/g, fecal coliforms from 0 to 5,3.102 CFU/g, and yeasts and moulds from 4 CFU/g to 1,1.103 CFU/g. No confirmed Salmonella and Escherichia coli colonies were detected. With respect to the microbiological evaluation, all the cooking flours showed satisfactory total aerobic flora, Escherichia ncoli and Salmonella, 64.71% showed satisfactory faecal coliforms and 94.12% showed satisfactory yeasts and moulds. All instant flours had satisfactory microbiological loads. Conclusion: overall, local infant flours sold in Ouagadougou are of satisfactory physicochemical and microbiological quality, with the exception of some cooking flours.
Subject(s)
Humans , Infant, Newborn , Infant , Child, Preschool , Child Nutrition Sciences , Flour , Diet, Food, and Nutrition , Nutritive ValueABSTRACT
BACKGROUND: This study was undertaken to identify and functionally characterize virulence genes from Salmonella isolates in street food and stool cultures. From February 2017 to May 2018, clinical and food Salmonella strains were isolated in three regions in Burkina Faso. Salmonella was serotyped according to the White-Kauffmann-Le Minor method, and polymerase chain reaction (PCR) was used to detec invA, spvR, spvC, fimA and stn virulence genes commonly associated with salmonellosis in Sub-Saharan Africa. RESULTS: A total of 106 Salmonella isolates (77 human stools; 14 sandwiches) was analyzed using a serological identification with an O-group test reagent. The presence of Salmonella was confirmed in 86% (91/106) of the samples were reactive (OMA-positive/OMB-positive). Salmonella serogroup O:4,5 was the most common serogroup detected (40%; 36/91). Salmonella Enteritidis and Typhimurium represented 5.5% (5/91) and 3.3% (3/91), respectively and were identified only from clinical isolates. Furthermore, 14 serotypes of Salmonella (12/91 human strains and 2/15 sandwich strains) were evocative of Kentucky/Bargny serotype. For the genetic profile, 66% (70/106) of the Salmonella had invA and stn genes; 77.4% (82/106) had the fimA gene. The spvR gene was found in 36.8% (39/106) of the isolates while 48.1% (51/106) had the spvC gene. Among the identified Salmonella Enteritidis and Salmonella Typhimurium isolated from stools, the virulence genes detected were invA (3/5) versus (2/3), fimA (4/5) versus (3/3), stn (3/5) versus (2/3), spvR (4/5) versus (2/3) and spvC (3/5) versus (2/3), respectively. CONCLUSION: This study reports the prevalence of Salmonella serotypes and virulence genes in clinical isolates and in street foods. It shows that food could be a significant source of Salmonella transmission to humans. Our results could help decision-making by the Burkina Faso health authority in the fight against street food-related diseases, in particular by training restaurateurs in food hygiene.
Subject(s)
Fast Foods/microbiology , Feces/microbiology , Salmonella/isolation & purification , Virulence Factors/genetics , Burkina Faso/epidemiology , Diarrhea/epidemiology , Diarrhea/microbiology , Genes, Bacterial , Humans , Prevalence , Salmonella/classification , Salmonella/genetics , Salmonella/pathogenicity , Serogroup , Serotyping , Virulence/geneticsABSTRACT
The palmyra palm Borassus aethiopum Mart. grow wild and gives natural stands in several localities of central-eastern and eastern regions of Burkina Faso. This work aimed to determine the nutritional, biochemical and microbiological composition of fresh palm sap from B. aethiopum Mart. during the first 4 days of tapping. The composition of palm sap was carried out by HPLC and standard methods. The sap collected during the first 4 days were sugary and contained less alcohol. The mean values of the pH, total and reducing sugars content were 4.84 ± 0.5, 11.36 ± 3.97 and 2.93 ± 1.22% w/v respectively. Sucrose, glucose, fructose and Vitamin C values were 6.75% w/v, 4.99 g/L, 7.09 g/L, 8.93% w/v respectively. Galactose and xylose were not detected. Soluble proteins, arabinose, phenols and ethanol were present in low concentration. Calcium, potassium, magnesium and ammonium were present in palm sap with highest potassium content (13.26 g/L). Lactate (2.41 ± 0.86 g/L), succinate (2.49 ± 1.46 g/L), acetate (0.01 ± 0.006 g/L), malate (0.17 ± 0.31 g/L), propionate (0.07 ± 0.04 g/L), citrate (0.19 ± 0.11 g/L), tartrate (0.08 ± 0.09 g/L) and pyruvate (0.05 ± 0.03 g/L) were detected in palm sap. The microbiological analysis of sap gave 1.23 ± 1.01 × 108 cfu/mL for total aerobic flora, 7.27 ± 1.19 × 105 cfu/mL for yeasts, 1.86 ± 1.63 × 107 cfu/mL for lactic acid bacteria and 3.75 ± 0.75 × 105 cfu/mL for acetic acid bacteria. The fresh sap from B. aethiopum presents good nutritional value and its consumption can help to improve dairy food intake of rural population. It can be used for the manufacture of various products like palm wine, syrups, sugars, functional foods, etc.
